CRISPETa

CRISPETa is a webserver (standalone version also available) for paired sgRNA design, such as required by DECKO. A flexible tool to design optimal pairs of sgRNAs for deletion of desired genomic regions. CRISPETa can be run on any number of targets – from one to thousands. At present, designs can be performed for 5 genomes: human, mouse, zebrafish, fruitfly and worm

Access it here: http://crispeta.crg.eu/

CRISPETa_webserver